Influencing factors of false negative in enzyme-linked immunosorbent assay

False negatives may be affected by the following factors:

1. Reagents on the production line have a rare "missing package", or the coated antigen or antibody is inactivated or has insufficient activity.

2. Improper transportation and storage of reagents. For example, the temperature during transportation is too high and the environment in which the reagents are stored is not good, resulting in reduced activity of the markers in the reagents, or the neutralization reagents are completely inactivated.

3. Errors during operation, errors, few or leaks during sample addition; slow addition of developer, observation time is late, so that the fading reaction appears false negative; fast, soaking, soaking Too long time, too many times of repeated washing, so that the reaction of immunomedicine education network collection and finishing complex is lost.

4. Ignore the role of the critical value serum and only act as a positive control in the test, thereby losing control of the critical point and causing false negatives.

5. Interpretation of results or misalignment when registering and sending reports, false negative results appearing.

Influencing factors of false negative in enzyme-linked immunosorbent assay

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